Anti-SERPINB1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30740

Core Product Specifications and Parameters

Biological Background: SERPINB1 Function and Localization

• SERPINB1 acts as a neutrophil serine protease inhibitor, playing an essential role in regulating the innate immune response, inflammation, and cellular homeostasis by protecting cells from proteases released during stress or infection. Related references: PMID:30692621

• It regulates the activity of neutrophil proteases including elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Related references: PMID:11747453 PMID:30692621

• SERPINB1 is a potent intracellular inhibitor of granzyme H (GZMH), directly blocking its proteolytic activity. Related references: PMID:23269243

• During inflammation, SERPINB1 limits the activity of inflammatory caspases CASP1, CASP4, and CASP5 by suppressing their CARD oligomerization and enzymatic activation. Related references: PMID:30692621

• When secreted, SERPINB1 promotes the proliferation of beta-cells via its protease inhibitory function. Related references: PMID:26701651

• Subcellular localization includes secretion, cytoplasm, cytolytic granules, and early endosomes.

• Tissue expression is highest in the neutrophil lineage within bone marrow, with intermediate levels in monocytes and low levels in lymphocytes, peaking at the promyelocyte stage.

• Post-translational modifications include acetylation and phosphorylation, and the protein belongs to the serpin family of serine protease inhibitors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-379 of human SERPINB1, covering the serpin domain; consider potential cross-reactivity with other serpin family members and validate specificity using appropriate controls.
• For Western blot, the predicted molecular weight is approximately 43 kDa; monitor for possible post-translational modifications that may cause band shifts.
• For IHC-P, antigen retrieval conditions may need optimization; validate with known positive and negative tissues.
• For IF/ICC, verify staining patterns in relevant cell types and include appropriate controls for subcellular localization.
• The antibody cross-reacts with human, mouse, and rat; confirm reactivity in your specific model system and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including this anti-SERPINB1 polyclonal antibody for studies in innate immunity and protease biology. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized procurement for rare-target raw materials, supporting your project from early development to clinical application.