Anti-Serotonin Transporter Polyclonal Antibody for WB, IHC-P, ELISA - Q60857

Core Product Specifications and Parameters

Biological Background: Serotonin Transporter Function and Localization

• The serotonin transporter (SERT), encoded by the Slc6a4 gene (also known as Htt or Sert), is a sodium-dependent transporter belonging to the solute carrier family 6, member 4. It acts as an antiporter, cotransporting serotonin with Na⁺ in exchange for K⁺, thus mediating the reuptake of serotonin from the synaptic cleft and limiting its signaling. Related references: PMID:9037532 PMID:9547354

• In the central nervous system, SERT is essential for serotonin homeostasis. During development, it controls serotonin uptake in glutamatergic neurons of the somatosensory cortex, guiding proper spatial organization of cortical neurons and sensory circuit formation. In the mature brain, it functions primarily in brainstem raphe neurons to terminate serotonin signaling by clearing the neurotransmitter from the synapse. Related references: PMID:25600870 PMID:9547354

• In the gastrointestinal tract, SERT expressed in enterocytes modulates mucosal serotonin levels through uptake and clearance, which is required for enteric neurogenesis and gastrointestinal reflexes. Related references: PMID:27111230

• SERT regulates blood serotonin levels by ensuring rapid, high-affinity uptake of serotonin from plasma into platelets, where it is stored in dense granules and later released upon stimulation. Related references: PMID:18317590

• The transport cycle involves a conformational transition from outward-open to inward-open states, driven by the binding of Na⁺, Cl⁻, and serotonin, and the intracellular binding of K⁺. Additionally, SERT exhibits a channel-like conductance for monovalent cations, mainly Na⁺, which is uncoupled from transport and may influence membrane potential.

• Subcellularly, SERT is localized to the plasma membrane, endomembrane system, endosome membrane, synaptic junctions, focal adhesions, and neuron projections.

• Tissue expression studies show SERT in the lung, midbrain, and brainstem, with prominent expression in brainstem raphe neurons. The protein undergoes post-translational modifications, including N-linked glycosylation and phosphorylation, and contains disulfide bonds. Related references: PMID:9037532 PMID:25600870

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-85) of mouse SERT. For Western blotting, a band at ~70 kDa is expected. Use reducing conditions and appropriate tissue lysates (e.g., brain or lung).
• For immunohistochemistry (paraffin-embedded sections), validate the antibody in known positive tissues (brain or gut) and optimize antigen retrieval conditions.
• As a polyclonal antibody, batch-to-batch consistency should be verified. Include appropriate positive and negative controls in all experiments.
• The antibody shows cross-reactivity with rat, making it suitable for comparative studies. Consider additional validation if used in other species.

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CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop platform for IVD raw material sourcing, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting projects in neurotransmitter transporter research and beyond. Whether you need serotonin transporter antibodies or rare target raw materials, CamelBio streamlines your development from concept to clinic.