Anti-SERCA2/ATP2A2 Rabbit Polyclonal Antibody for WB, IHC-P - P16615

Core Product Specifications and Parameters

Biological Background: SERCA2/ATP2A2 Function and Localization

• Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2), also known as calcium pump 2, is encoded by the ATP2A2 gene and belongs to the P-type cation transport ATPase family. Alternative splicing generates multiple isoforms with tissue-specific expression.
• SERCA2 is a magnesium-dependent enzyme that catalyzes ATP hydrolysis coupled with the translocation of calcium ions from the cytosol into the sarcoplasmic or endoplasmic reticulum lumen, essential for intracellular calcium homeostasis. Related references: PMID:12542527 PMID:16402920
• This pump plays a key role in the contraction/relaxation cycle of muscle cells by regulating calcium reuptake into the sarcoplasmic reticulum.
• Beyond muscle, SERCA2 is involved in autophagy: upon interaction with VMP1, it controls ER-isolation membrane contacts necessary for autophagosome formation, and modulates contacts with lipid droplets, mitochondria, and endosomes. Related references: PMID:28890335
• In osteoclasts, SERCA2 acts as a regulator of TNFSF11-mediated calcium signaling via interaction with TMEM64, promoting NFATC1 activation and mitochondrial ROS generation critical for osteoclast differentiation.
• Subcellularly, SERCA2 localizes to the endoplasmic reticulum membrane and sarcoplasmic reticulum membrane, reflecting its role in intracellular calcium storage and signaling.
• Tissue expression varies: isoform 1 is widely expressed in smooth muscle and nonmuscle tissues (high in liver, pancreas, lung), while isoforms 2 and 3 are predominantly found in cardiac and slow-twitch skeletal muscle.
• Key post-translational modifications include nucleotide-binding, phosphorylation, and disulfide bonds; the protein is also subject to nitration, as indicated by multiple phosphoproteomic studies.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment spanning amino acids 111-253 of human SERCA2/ATP2A2 (conserved region). It may recognize multiple isoforms and cross-react with mouse and rat SERCA2 as confirmed.
• For Western blot, expect a band around 115 kDa. Optimize reducing/non‑reducing conditions and consider loading controls from tissues with high SERCA2 expression (e.g., liver, heart).
• For immunohistochemistry (paraffin sections), employ appropriate antigen retrieval methods and validate staining in tissues known to express SERCA2. Include positive and negative tissue controls.
• For ELISA applications, use the recombinant immunogen or purified protein as coating antigen. Verify coating conditions and antibody dilution series.
• As with any polyclonal antibody, lot‑to‑lot consistency should be verified for quantitative assays. Consider performing pilot experiments when switching batches.

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