Anti-SERCA2/ATP2A2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16615

Core Product Specifications and Parameters

Biological Background: SERCA2/ATP2A2 Function and Localization

• Encoded by the ATP2A2 gene, SERCA2 (also known as calcium pump 2) is a P-type ATPase responsible for transporting calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen, a process essential for muscle relaxation and cellular calcium homeostasis (PubMed:12542527, PubMed:16402920). Related references: PMID:12542527, PMID:16402920
• Multiple isoforms exist: isoform 1 is widely expressed in smooth muscle and nonmuscle tissues, while isoforms 2 and 3 are highly expressed in cardiac and slow-twitch skeletal muscle; isoform 3 shows restricted expression in cardiomyocytes.
• The protein resides in the endoplasmic reticulum and sarcoplasmic reticulum membranes, where it functions as a translocase with ten transmembrane helices.
• SERCA2 plays a role in autophagy by mediating ER-isolation membrane contacts for autophagosome formation upon starvation, through interaction with VMP1 (PubMed:28890335). Related references: PMID:28890335
• It also contributes to osteoclast differentiation by interacting with TMEM64 to generate calcium spikes that activate NFATC1 and promote mitochondrial ROS production.
• Key structural features include an ATP-binding domain, multiple metal-binding sites (magnesium, calcium), and post-translational modifications such as phosphorylation and nitration, as evidenced by proteomics studies.
• The protein is associated with disease variants linked to Darier disease and epilepsy, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-80) of human SERCA2, a conserved region; consider sequence alignment with mouse and rat to verify epitope similarity for cross-reactivity assays.
• For Western blot, a band around 115 kDa is expected; however, due to potential post-translational modifications or isoform variations, additional bands may appear, so validate using appropriate lysates and controls.
• In IHC-P and IF/ICC, fixative choice (e.g., 4% PFA vs. methanol) may impact antibody binding; optimize for the specific tissue or cell type, with antigen retrieval recommended for paraffin sections.
• The antibody is applicable in ELISA; ensure proper coating and blocking conditions to minimize background.
• Lot-specific validation is recommended for each new application, as polyclonal antibodies can vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for immunoassay development. For calcium signaling and muscle physiology research, our one-stop sourcing supports diagnostic manufacturers and labs from proof-of-concept to clinic, with expertise in rare target antibody production and bulk supply capabilities. Partner with CamelBio to streamline your raw material procurement for SERCA2/ATP2A2 and related targets.