Anti-SERCA2/ATP2A2 Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P16615

Core Product Specifications and Parameters

Biological Background: SERCA2/ATP2A2 Function and Localization

• SERCA2, encoded by ATP2A2, is a magnesium-dependent calcium-transporting ATPase that couples ATP hydrolysis to translocation of calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen (PubMed:12542527, PubMed:16402920). Related references: PMID:12542527 PMID:16402920
• It plays a key role in muscle contraction/relaxation by regulating cytosolic calcium levels and is essential for calcium homeostasis in non-muscle tissues.
• SERCA2 has three main isoforms generated by alternative splicing: isoform 1 widely expressed in smooth muscle and nonmuscle tissues; isoforms 2 and 3 predominantly in heart and slow-twitch skeletal muscle.
• In response to starvation, SERCA2 becomes involved in autophagy by interacting with VMP1 to control ER-isolation membrane contacts for autophagosome formation; it also modulates ER contacts with lipid droplets, mitochondria, and endosomes (PubMed:28890335). Related references: PMID:28890335
• SERCA2 is located in the endoplasmic reticulum and sarcoplasmic reticulum membranes.
• It is a critical regulator of osteoclast differentiation via TNFSF11-mediated Ca(2+) signaling, interacting with TMEM64 to drive NFATC1 activation and mitochondrial ROS production.
• Keywords include alternative splicing, calcium transport, ATP-binding, magnesium, metal-binding, transmembrane, and disulfide bond; post-translational modifications include nitration and phosphorylation.
• Tissue specificity: Isoform 1 is highest in liver, pancreas, lung; intermediate in brain, kidney, placenta; lower in heart and skeletal muscle. Isoforms 2 and 3 are cardiac/slow-twitch muscle-specific.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 943-1042) targets the C-terminal region of SERCA2. Given the high sequence similarity among isoforms, consider possible detection of multiple isoforms depending on sample type.
• For WB, an expected band around 115 kDa should be observed; validate specificity using knockout controls or siRNA knockdown experiments.
• For IF/ICC, optimize fixation and permeabilization protocols for the target cell type; note that SERCA2 localizes to ER/SR membranes, so staining may appear reticular.
• In IP and ELISA, confirm antibody performance with recombinant protein or endogenous samples; titration may be necessary to avoid high background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research labs seeking high-quality IVD raw materials. This SERCA2/ATP2A2 antibody supports investigations into calcium signaling, muscle physiology, and ER stress pathways. Beyond individual antibodies, CamelBio supplies validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and can assist with sourcing rare targets—helping you streamline assay development from concept to clinic.