Anti-SERCA1/ATP2A1 Polyclonal Antibody for WB - O14983

Core Product Specifications and Parameters

Biological Background: SERCA1/ATP2A1 Function and Localization

• Acts as the major Ca2+ ATPase in fast-twitch skeletal muscle, responsible for reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum, a process critical for muscle relaxation.
  Related references: PMID:10914677
• Catalyzes ATP hydrolysis coupled with calcium translocation, providing the energy for calcium transport across the sarcoplasmic reticulum membrane.
• Encoded by the ATP2A1 gene; alternative splicing generates multiple isoforms with distinct regulation.
• Localizes to the sarcoplasmic reticulum and endoplasmic reticulum membranes, with multiple transmembrane helices.
• Exhibits tissue specificity for skeletal muscle, particularly fast twitch (type II) fibers.
• Contains ATP-binding, calcium-binding, and magnesium-binding sites essential for its catalytic activity.
• Regulated by phosphorylation; classified as a P-type ATPase involved in calcium transport and ion homeostasis.
• Mutations are associated with Brody myopathy, a disorder of muscle relaxation.

Experimental Guidance and Technical Tips

• The immunogen is designed against the C-terminal region (aa 901–1001) of human SERCA1; verify if the antibody recognizes relevant splice variants or isoforms in your experimental system.
• For Western blot, expect a band at approximately 110 kDa. Optimize transfer conditions (e.g., extended transfer time, lower methanol) for efficient blotting of high-molecular-weight proteins.
• Cross-reactivity with mouse is confirmed; consider performing validation if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined channel to IVD raw materials, from optimized polyclonal antibodies like this SERCA1/ATP2A1 Rabbit pAb to complementary ancillary reagents. We support your calcium transport and muscle research projects with reliable supply and can assist in sourcing rare-target raw materials, validated pairs, and bulk orders to accelerate your assay development.