Anti-Septin 8 Rabbit Monoclonal Antibody for WB and ELISA - Q92599

Core Product Specifications and Parameters

Biological Background: Septin 8 Function and Localization

• Septin-8 is a member of the septin family of filament-forming cytoskeletal GTPases, encoded by the SEPTIN8 gene.
• Alternative splicing produces multiple isoforms (isoform 1, 2, and 3) that display tissue-specific expression patterns.
• Septin-8 is involved in platelet secretion, as suggested by functional studies. Related references: PMID:15116257
• It participates in SNARE complex formation in synaptic vesicles, facilitating neurotransmitter release (By similarity).
• Septin-8 stabilizes BACE1 protein levels and promotes BACE1 sorting to recycling/endosomal compartments, thereby modulating amyloid-beta precursor protein (APP) processing.
• Subcellularly, Septin-8 localizes to the cytoplasm, cytoskeleton, synaptic vesicles, and presynaptic terminals, with presence in axons.
• Post-translational modifications include acetylation and phosphorylation, consistent with its dynamic regulation.
• Septin-8 is widely expressed, with highest abundance in aorta; isoform-specific expression is observed in brain regions and reproductive tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human Septin-8; for WB, validate detection in lysates from tissues with high expression such as brain or heart.
• For ELISA applications, consider optimizing the coating antigen concentration and pairing with a detection antibody to enhance sensitivity.
• Given the role of Septin-8 in BACE1 trafficking, this antibody may be useful in Alzheimer's disease research, but validation in relevant cellular models is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise in rare target sourcing ensures reliable supply of Septin-8 antibodies and related reagents for diagnostic and research applications in neuroscience and cardiovascular biology.