Anti-Septin 7 Polyclonal Antibody for WB, IHC-P, ELISA - Q16181

Core Product Specifications and Parameters

Biological Background: Septin-7 Function and Localization

• Septin-7 (CDC10 protein homolog) is a member of the septin family of filament-forming cytoskeletal GTPases, involved in actin cytoskeleton organization.
• It is required for normal mitosis progression, including cytokinesis and proper association of CENPE with the kinetochore.
• Subcellularly, Septin-7 localizes to the cytoplasm, centromere/kinetochore, spindle, cleavage furrow, and midbody during cell division; it is also present in cilium axoneme and flagellum.
• Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2, and likely SEPTIN4 at the sperm annulus, essential for sperm tail integrity and motility during postmeiotic differentiation. Related references: PMID:25588830
• The protein undergoes post-translational acetylation and phosphorylation, and is widely expressed across human tissues.
• Key functional keywords: GTP-binding, nucleotide-binding, coiled coil, mitosis, cell cycle, cell division, cilium, flagellum, spermatogenesis, differentiation.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal domain (aa 307-436) of human Septin-7, which shares high sequence homology with mouse and rat; this likely accounts for the observed cross-reactivity. Consider using this antibody for endogenous Septin-7 detection across these species.
• For Western blotting, validate with appropriate positive control lysates (e.g., HeLa, HEK293) and confirm the expected band at ~51 kDa. Due to widespread expression, many cell lines may be suitable.
• For immunohistochemistry (paraffin), antigen retrieval optimization is recommended. Validate staining specificity by comparing with negative controls (e.g., knockout tissues or blocking peptides if available).
• In ELISA, confirm specificity through recombinant protein titration and ensure proper assay conditions. Cross-reactivity with other septin family members should be experimentally excluded.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-Septin-7 polyclonal antibody supports research into cytoskeletal dynamics, mitosis, and ciliogenesis. As a comprehensive sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and rare target raw-material procurement to accelerate your assay development from concept to clinic.