Anti-Septin 5 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99719

Core Product Specifications and Parameters

Biological Background: Septin 5 Function and Localization

• Septin 5 (also known as CDCrel-1, Peanut-like protein 1) is a filament-forming cytoskeletal GTPase belonging to the septin family.
• It may play a role in cytokinesis, the final step of cell division.
• The protein may also be involved in platelet secretion, a critical process in hemostasis and thrombosis.
• Septin 5 localizes to the cytoplasm and associates with the cytoskeleton.
• It is expressed at high levels in the central nervous system, heart, and platelets.
• Post-translational modifications include methylation and phosphorylation, which may regulate its function.
• Alternative splicing generates multiple isoforms of Septin 5.
• The predicted molecular weight is approximately 43 kDa, consistent with the UniProt-calculated mass of 42,777 Da.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a specific band around 43 kDa; use appropriate loading controls for quantification.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; validate in tissues with known high expression, such as brain or heart.
• For immunofluorescence (IF/ICC), optimize permeabilization conditions to detect the cytoplasmic and cytoskeletal protein.
• As a polyclonal antibody, consider lot-to-lot consistency and include positive and negative controls in each experiment.
• Because the immunogen covers the full-length recombinant protein, the antibody may cross-react with other septin family members; validate specificity via knockdown or blocking experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of high-quality IVD raw materials, including antibodies like this anti-Septin 5 polyclonal antibody for research into cytoskeletal dynamics, cell division, and platelet biology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. With a comprehensive supply chain and technical expertise, CamelBio ensures you have a reliable partner for even rare target raw-material sourcing.