Anti-Septin 4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43236

Core Product Specifications and Parameters

Biological Background: Septin 4 Function and Localization

• Septin 4 is a filament-forming cytoskeletal GTPase, also known as Bradeion beta, Brain protein H5, and hCDCREL-2. It belongs to the septin family of proteins involved in cell division and cytoskeletal organization.
• Pro-apoptotic protein: mediates TGF-beta-induced apoptosis (PubMed:11146656, PubMed:15837787) and induces cell death by binding and inhibiting XIAP, leading to caspase activation (PubMed:15029247). Related references: PMID:11146656, PMID:15837787, PMID:15029247
• Positively regulates ubiquitination and degradation of BCL2 via interaction with XIAP, promoting apoptosis (PubMed:29020630). Related references: PMID:29020630
• Essential for male fertility and sperm motility by establishing the sperm flagellum annulus, a fibrous ring critical for structural integrity (PubMed:36135717). Related references: PMID:36135717
• Involved in neuronal development: migration of cortical neurons and formation of leading processes; also regulates dopaminergic metabolism as a presynaptic scaffold protein (By similarity).
• Subcellular localization includes cytoplasm, cell projections (cilium, flagellum, axon, dendrite), mitochondrion, nucleus, and cytoplasmic vesicles.
• Widely expressed in adult and fetal tissues, with highest in brain, heart, liver, adrenal gland; detected in dopaminergic neuron presynaptic terminals and colonic crypts (PubMed:17296554, PubMed:30389919). Related references: PMID:17296554, PMID:30389919
• Keywords: Alternative splicing, Coiled coil, GTP-binding, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–100 amino acids of human Septin 4, suggesting the antibody targets the N-terminal domain; consider validating specificity with peptide blocking or knockdown/knockout controls.
• For Western blot (WB), a band around 55 kDa is expected; verify using positive controls such as HeLa or HEK293 cell lysates and optimize loading amounts to avoid overexposure.
• For immunohistochemistry (IHC-P), test on formalin-fixed paraffin-embedded human brain or testis sections; heat-induced antigen retrieval in citrate buffer (pH 6.0) may improve staining.
• For immunofluorescence/immunocytochemistry (IF/ICC), check localization in cultured cell lines; fixation with 4% PFA and permeabilization with 0.1% Triton X-100 are typical starting points.
• ELISA: Suitable as a capture or detection reagent; pairing with another Septin 4 antibody (if available) may enhance sensitivity.
• Cross-reactivity with mouse and rat is reported; validate in the relevant sample system to confirm species-specific reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for apoptosis, spermatogenesis, and neuroscience research. Our integrated supply chain streamlines assay development from concept to clinic, ensuring consistent quality and rapid access to critical raw materials. Leverage our expertise to accelerate your Septin 4-related project timelines.