Anti-Septin 3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UH03

Core Product Specifications and Parameters

Biological Background: Septin 3 Function and Localization

• Septin 3 is a neuronal-specific member of the septin family of filament-forming cytoskeletal GTPases.
• It may play a role in cytokinesis, the final step of cell division.
• The protein localizes to the cytoplasm and cytoskeleton, and is also found at synapses.
• It is specifically expressed in the brain, indicating a role in neuronal function.
• Alternative splicing generates multiple isoforms of Septin 3, potentially with different functions.
• The protein is subject to phosphorylation, as indicated by the "Phosphoprotein" keyword.
• Keywords: 3D-structure, Alternative splicing, Cell cycle, Cell division, Cytoplasm, Cytoskeleton, GTP-binding, Nucleotide-binding, Phosphoprotein, Proteomics identification, Reference proteome, Synapse.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 179-358 of human Septin 3. Ensure your target region is present in the isoform of interest.
• For Western blotting, the expected band size is approximately 41 kDa; however, validate in your sample system as post-translational modifications or alternative splicing may shift migration.
• For immunofluorescence/immunocytochemistry, expect cytoplasmic and cytoskeletal staining; synaptic localization may require high-resolution imaging.
• This antibody has been validated for ELISA; consider it for quantitative assays after appropriate optimization.
• As a polyclonal antibody, it may recognize multiple epitopes; cross-adsorption might improve specificity if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs and bulk ancillary reagents. Our portfolio includes optimized polyclonal and monoclonal antibodies for neuroscience research targets such as Septin 3, supporting applications from Western blotting to ELISA. For rare or custom targets, we offer specialized sourcing and technical consultation to accelerate your project from concept to clinic.