Anti-Septin 2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q15019

Core Product Specifications and Parameters

Biological Background: Septin 2 Function and Localization

• Septin-2 is a member of the septin family of filament-forming cytoskeletal GTPases and is encoded by the SEPTIN2 gene. Alternative names include NEDD-5 and DIFF6.
• It forms filamentous structures with other septins (e.g., SEPTIN12, SEPTIN6, SEPTIN4) at the sperm annulus, essential for sperm tail structural integrity and motility during postmeiotic differentiation.
  Related references:
  PMID:25588830
• During mitosis, SEPT2 forms a scaffold at the spindle midplane that maintains CENPE localization at kinetochores, ensuring chromosome congression; it also contributes to chromosome segregation and spindle elongation in anaphase.
• In ciliated cells, SEPT2 is required for the integrity of the diffusion barrier at the base of primary cilia, regulating the assembly of the tectonic-like complex (B9 complex) via TMEM231 protein localization.
• Subcellular localization includes cytoplasm, cytoskeleton, mitotic spindle, cleavage furrow, midbody, cell cortex, and the base of cilia/flagella, reflecting its diverse structural and regulatory roles.
• Septin-2 is widely expressed across human tissues, with notably documented expression in the liver.
  Related references:
  PMID:15915442
  PMID:19165576
• Key post-translational modifications include acetylation and phosphorylation, and the protein binds GTP, consistent with its regulatory role in cytoskeletal dynamics.
• SEPT2 may also participate in the internalization of intracellular microbial pathogens such as Listeria monocytogenes and Shigella flexneri.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 112–361 of human SEPT2 (NP_004395.1), which lies within the conserved GTPase domain; validation is recommended in systems expressing full-length SEPT2.
• For Western blotting (WB), the predicted molecular weight is approximately 41 kDa; however, due to potential post-translational modifications, migration may shift slightly—consider using appropriate positive control lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider SEPT2’s association with the mitotic spindle, midbody, and ciliary structures; fixation and permeabilization protocols should be optimized to preserve these dynamic filamentous structures.
• Cross-reactivity is documented for human and mouse; for other species, sequence homology analysis of the immunogen region is advised before experimental use.
• As a polyclonal antibody, lot-to-lot consistency in titer and specificity is inherent but may benefit from internal validation for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams by providing streamlined access to validated IVD raw materials, including high-quality recombinant antibodies and ancillary reagents. This anti-Septin 2 polyclonal antibody supports cell division and cytoskeleton research areas, and CamelBio’s extensive portfolio can supply complementary antibody pairs, optimized monoclonal/polyclonal options, and bulk quantities to accelerate your assay development. For rare or custom targets, our expert sourcing capabilities ensure reliable supply continuity from concept to clinic.