Anti-Septin 11 Rabbit Polyclonal Antibody for WB, ELISA - Q9NVA2

Core Product Specifications and Parameters

Biological Background: Septin-11 Function and Localization

• Septin-11 is a filament-forming cytoskeletal GTPase encoded by the SEPTIN11 gene (also known as SEPT11), belonging to the septin family of proteins involved in cytokinesis and cytoskeletal organization.
• The protein plays a putative role in cytokinesis (cell division) and contributes to the structural integrity of the cytoskeleton.
• In neurons, Septin-11 is implicated in dendritic arborization, dendritic spine morphogenesis, and GABAergic synaptic connectivity, suggesting a key role in neuronal cytoarchitecture.
• Subcellularly, it localizes to the cytoplasm, cytoskeleton, synapses, dendritic spines, and axons, reflecting diverse intracellular distributions.
• Septin-11 undergoes post-translational modifications including acetylation and phosphorylation, and contains a coiled-coil domain that mediates protein-protein interactions.
• It is widely expressed across tissues but notably absent in leukocytes, indicating a tissue-specific expression pattern.
• The protein possesses a GTP-binding domain and exhibits GTPase activity, a hallmark of septins, and is involved in nucleotide binding.
• During Listeria monocytogenes infection, Septin-11 restricts the efficacy of bacterial entry, suggesting a role in host defense.
• The gene is subject to alternative splicing and chromosomal rearrangements, potentially generating multiple isoforms, and its 3D structure has been resolved.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering the N-terminal region (amino acids 1-120) of human Septin-11. Consider validating reactivity in your species of interest (human, mouse, rat) via Western blot using appropriate tissue or cell lysates.
• For Western blotting, the predicted molecular weight is approximately 49 kDa. Use suitable gel percentage and transfer conditions; inclusion of a positive control (e.g., brain tissue lysate) is recommended.
• For ELISA, test a range of antibody dilutions to optimize signal-to-noise ratio. The antibody’s cross-reactivity with mouse and rat antigens suggests broad utility, but assay-specific validation is advised.
• As a polyclonal antibody, it may detect multiple epitopes; peptide blocking experiments can confirm binding specificity.
• Although not validated for other applications, this antibody may be useful in immunofluorescence or immunohistochemistry for cytoskeletal targets—if attempting, optimize fixation and permeabilization protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with streamlined access to high-quality IVD raw materials, covering every stage from concept to clinic. For cytoskeletal and neuronal research applications, this anti-Septin-11 polyclonal antibody supports Western blot and ELISA workflows across human, mouse, and rat samples. Additionally, we offer comprehensive sourcing for validated antibody pairs, bulk ancillary reagents, and custom raw-material solutions for rare targets, ensuring your projects stay on track.