Anti-Septin 11 Polyclonal Antibody for WB, ELISA - Q9NVA2

Core Product Specifications and Parameters

Biological Background: Septin-11 Function and Localization

• Septin-11 is a filament-forming cytoskeletal GTPase belonging to the septin family.
• It may play a role in cytokinesis (cell division), though this is not fully established.
• In neurons, it contributes to cytoarchitecture, including dendritic arborization and dendritic spine formation, and supports GABAergic synaptic connectivity (by similarity).
• During Listeria monocytogenes infection, Septin-11 restricts bacterial entry efficacy.
• The protein localizes to the cytoplasm, cytoskeleton, and is enriched at synapses, dendritic spines, and axons.
• It is widely expressed in tissues, except leukocytes.
• Post-translational modifications include acetylation and phosphorylation; multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-120), ensuring recognition of full-length Septin-11 and common isoforms.
• For Western blot, consider using standard starting dilutions (e.g., 1:500–1:2000) with lysates from human, mouse, or rat cells; HeLa or neuronal cell lines are suitable based on expression patterns.
• In ELISA, direct or indirect coating may be tested; owing to the polyclonal nature, confirm batch-to-batch consistency with a reference standard.
• For functional studies involving Listeria infection, note that Septin-11 negatively regulates entry efficacy; validate specificity in knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a one‑stop channel for IVD raw materials, covering the entire development pipeline. This Septin-11 polyclonal antibody supports studies in cytoskeletal dynamics, cell division, and neuroscience, and complements our portfolio of optimized antibodies, validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Our supply framework is designed to streamline your procurement and scale your projects efficiently.