Anti-SEPSECS Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9HD40

Core Product Specifications and Parameters

Biological Background: SEPSECS Function and Localization

• SEPSECS (Sep(SecS)) is a pyridoxal phosphate-dependent transferase that converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec), an essential step for selenoprotein biosynthesis.
• It is also known as the SLA/LP autoantigen, recognized by autoantibodies in autoimmune hepatitis, and as soluble liver antigen (SLA) or liver-pancreas antigen (LP).
• The enzyme localizes to the cytoplasm where it matures the tRNA carrying selenocysteine, the 21st amino acid.
• SEPSECS is primarily expressed in the liver, pancreas, kidney, and lung, with increased expression in PHA-stimulated T-cells.
• It is a phosphoprotein and a tRNA-binding protein, reflecting its role in protein synthesis machinery regulation.
• Mutations in the SEPSECS gene are implicated in pontocerebellar hypoplasia type 2D (PCH2D), a severe neurodegenerative disorder characterized by intellectual disability and epilepsy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 222–501, spanning a conserved region suitable for detecting endogenous SEPSECS across human, mouse, and rat samples.
• For Western blot, expect a band around 56 kDa; consider using liver, pancreas, or kidney lysates as positive controls based on tissue expression profiles.
• Validate antibody specificity in your experimental setup, especially when working with overexpressed or stimulated cell models (e.g., PHA-treated T-cells) that upregulate SEPSECS.
• For IF/ICC, fix and permeabilize cells appropriately; cytoplasmic staining pattern should be observed.
• Optimize ELISA conditions with recombinant SEPSECS protein or peptide as coating antigen to establish a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive IVD raw material solutions. This anti-SEPSECS polyclonal antibody, validated for multiple applications, is available alongside paired reagents, recombinant proteins, and bulk ancillary materials to accelerate your selenoprotein-related research or diagnostic assay development. Contact us for custom sourcing of rare targets or validated antibody pairs to meet your clinical and R&D needs.