Anti-SEPN1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NZV5

Core Product Specifications and Parameters

Biological Background: SEPN1 Function and Localization

• Selenoprotein N (SEPN1) is an endoplasmic reticulum membrane protein that protects cells from oxidative stress and regulates redox-related calcium homeostasis.
• It protects the ER calcium pump ATP2A2 from ERO1A-mediated oxidative damage by reducing cysteinyl sulfenic acid to free thiol, restoring pump activity. Related references: PMID:25452428
• Acts as a modulator of ryanodine receptor (RyR) activity, preserving RyR from oxidation or directly controlling its redox state to regulate calcium mobilization required for normal muscle development and differentiation. Related references: PMID:18713863, PMID:19557870
• Essential for muscle regeneration and satellite cell maintenance in skeletal muscle. Related references: PMID:21131290
• Isoform 1 and isoform 2 are expressed in skeletal muscle, brain, lung, and placenta; isoform 2 is additionally found in heart, diaphragm, and stomach.
• Keywords from UniProt include: Alternative splicing, Desmin-related myopathy, Disease variant, Endoplasmic reticulum, Glycoprotein, Membrane, Myofibrillar myopathy, Oxidoreductase, Selenocysteine, Signal.
• The calculated molecular weight is approximately 66 kDa (UniProt: 65.8 kDa), consistent with the observed band size.

Experimental Guidance and Technical Tips

• This antibody is raised against a recombinant fragment (aa 341-590) of human SEPN1; interpret specificity in the context of this immunogen region.
• For Western blot, the expected band is ~66 kDa. Validate positivity using human or mouse tissue/cell lysates known to express SEPN1 (e.g., skeletal muscle, lung).
• For ELISA, optimize coating antigen and antibody dilutions; the recombinant immunogen may serve as a positive control.
• As an ER membrane protein, efficient extraction may require a strong lysis buffer (e.g., RIPA) with sonication to solubilize SEPN1.
• When working with mouse samples, confirm reactivity based on sequence homology and provided cross-reactivity data.

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