Anti-SENP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9HC62

Core Product Specifications and Parameters

Biological Background: SENP2 Function and Localization

• SENP2 (Sentrin-specific protease 2) is a SUMO-specific protease that catalyzes both the maturation of SUMO propeptides and the deconjugation of SUMO from modified proteins. Related references: PMID:11896061 PMID:12192048 PMID:15296745
• It hydrolyzes the C-terminal peptide bonds of SUMO1, SUMO2, and SUMO3 to generate mature forms, a prerequisite for SUMO conjugation. Related references: PMID:15296745
• SENP2 deconjugates SUMO2 from MTA1, modulating its function in gene regulation. Related references: PMID:21965678
• It desumoylates and stabilizes CEBPB, promoting adipocyte differentiation. Related references: PMID:20194620
• SENP2 may down-regulate CTNNB1 levels, thereby modulating the Wnt signaling pathway (By similarity).
• It localizes to the nucleus, nuclear pore complex, nuclear membrane, and cytoplasm, consistent with its roles in nucleocytoplasmic transport and SUMO processing.
• The protein is a thiol protease that undergoes phosphorylation, and its activity is critical for maintaining SUMO homeostasis.
• It also acts as a regulator of the cGAS-STING pathway by desumoylating CGAS and STING1 during viral infection (By similarity).

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human, mouse, or rat cell lines known to express SENP2; the expected band is approximately 68kDa.
• The immunogen (amino acids 366–589) overlaps with the C-terminal region; detection of potential cleavage products should be verified.
• For IHC paraffin sections, antigen retrieval may be necessary; optimization of retrieval conditions and antibody dilution is recommended.
• In ELISA, evaluate both direct and indirect formats, and include negative controls to assess specificity.
• Cross-reactivity with homologous SUMO proteases (e.g., SENP1, SENP3) is possible; validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for high-quality IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents for diagnostic development. This anti-SENP2 polyclonal antibody supports research in SUMOylation, Wnt signaling, and cancer biology. Leverage our comprehensive portfolio to streamline your assay development from concept to clinic.