Anti-SENP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9P0U3

Core Product Specifications and Parameters

Biological Background: SENP1 Function and Localization

• Sentrin-specific protease 1 (SENP1), also known as SuPr-2, is a protease that catalyzes two essential functions in the SUMO pathway: hydrolysis of alpha‑linked peptide bonds at the C‑terminus of SUMO propeptides (SUMO1, SUMO2, SUMO3) to generate mature forms, and deconjugation of SUMO from target proteins by cleaving epsilon‑linked peptide bonds between the C‑terminal glycine of mature SUMO and lysine residues on substrates. Related references: PMID:10652325, PMID:15199155, PMID:15487983
• Key deSUMOylation substrates include HIPK2, HDAC1, BHLHE40/DEC1, CLOCK, MTA1, METTL3, ALKBH5, CCAR2, and GPS2, linking SENP1 to transcriptional control, RNA methylation, and DNA damage responses. Related references: PMID:16253240, PMID:15199155, PMID:21829689, PMID:23160374, PMID:21965678, PMID:29506078, PMID:34048572, PMID:37257451, PMID:25406032, PMID:24943844
• SENP1 localizes to both the nucleus and cytoplasm, enabling it to regulate a broad range of SUMO‑dependent processes in these compartments.
• It is highly expressed in testis, with lower levels detected in thymus, pancreas, spleen, liver, ovary, and small intestine, suggesting tissue‑specific roles in SUMO pathway modulation.
• SENP1 is a phosphoprotein that undergoes alternative splicing; it belongs to the Ulp1 protease family and functions as a thiol protease (hydrolase). Its canonical molecular weight is approximately 73 kDa (73.4 kDa calculated from Uniprot).

Experimental Guidance and Technical Tips

• For Western blotting, expect a specific band around 73 kDa; validate using whole‑cell lysates from human (HeLa, HEK293), mouse, or rat cell lines and tissues where SENP1 is expressed (e.g., testis).
• For immunohistochemistry (IHC‑P), consider antigen retrieval with citrate buffer (pH 6.0) and validate staining in formalin‑fixed, paraffin‑embedded testis or other positive control tissues.
• In ELISA applications, a recombinant SENP1 protein standard may be used to establish assay sensitivity and specificity.
• Because this is a polyclonal antibody, each lot may exhibit slight variations in titer; it is advisable to determine the optimal dilution empirically in your experimental system.
• Cross‑reactivity with mouse and rat has been reported, but always confirm specificity in the relevant species background using appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing IVD raw materials, bridging the gap between discovery and clinical application. For researchers investigating SUMOylation, deSUMOylation, and related epigenetic mechanisms, we offer reliable access to this SENP1 polyclonal antibody along with a complete portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Let CamelBio streamline your procurement process and support your assay development from concept to clinic.