Anti-SEMG1 Rabbit Polyclonal Antibody for WB, ELISA - P04279

Core Product Specifications and Parameters

Biological Background: SEMG1 Function and Localization

• Semenogelin-1 (SEMG1) is the predominant protein in human semen, playing a critical role in the formation of a gel matrix that entraps accessory gland secretions and ejaculated spermatozoa.
• Upon proteolytic cleavage by prostate-specific antigen (KLK3/PSA), SEMG1 fragments contribute to the activation of progressive sperm motility.
• Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone (FSH), providing a feedback mechanism.
• Encoded by the SEMG1 gene on chromosome 20, alternative splicing produces multiple isoforms, which may have distinct functional properties.
• SEMG1 is synthesized and secreted exclusively by the seminal vesicle, as indicated by tissue specificity data.
• The protein contains a signal peptide, disulfide bonds, and a pyrrolidone carboxylic acid modification at the N-terminus, typical of secreted proteins.
• Classified as a cancer/testis antigen (CT103), SEMG1 may have implications in reproductive immunology and cancer biology.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 24-310, excluding the signal peptide, suggesting the antibody recognizes the mature secreted protein and its proteolytic fragments.
• For Western blot, the predicted molecular weight is ~52 kDa (UniProt: 52131 Da); however, multiple bands may be observed in semen or tissue lysates due to natural proteolytic processing by KLK3/PSA.
• When using ELISA, consider coating with recombinant SEMG1 or its fragments, and validate detection conditions in biological matrices such as seminal plasma.
• Cross-reactivity with mouse and rat SEMG1 orthologs has been reported, but sequence homology should be examined to anticipate potential variations in epitope recognition; validation in the target species is recommended.
• This polyclonal antibody may be suitable for immunohistochemistry or immunofluorescence, though these applications have not been explicitly validated; optimization with appropriate controls is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for reproductive health diagnostics. As a dedicated sourcing partner, we facilitate access to rare target reagents like SEMG1 antibodies, supporting research into semen analysis and fertility biomarkers from concept to clinic.