Anti-SEMG1 Polyclonal Antibody for WB, ELISA - P04279

Core Product Specifications and Parameters

Biological Background: Semenogelin-1 Function and Localization

• SEMG1 (Semenogelin-1) is the predominant protein in human semen, encoded by the SEMG1 gene, with reported isoforms of approximately 45 kDa and 52 kDa.
• The protein participates in the formation of a gel matrix that entraps accessory gland secretions and ejaculated spermatozoa, and its fragmentation by KLK3/PSA activates progressive sperm movements.
• Proteolytic degradation of semenogelin generates alpha-inhibin-92 and alpha-inhibin-31, which inhibit pituitary follicle-stimulating hormone (FSH) secretion.
• SEMG1 is a secreted protein, predominantly expressed in the seminal vesicle.
• Post-translational modifications include disulfide bonds and pyrrolidone carboxylic acid; the 3D structure of the protein has been resolved.
• SEMG1 is also designated as cancer/testis antigen 103 (CT103), suggesting potential ectopic expression in certain malignancies.
• Alternative splicing of the SEMG1 gene may contribute to protein diversity, as indicated by the presence of multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 312-462) of human SEMG1, which may improve specificity for the full-length protein and its cleavage products.
• For Western blot, consider using the antibody to detect bands around 45 kDa and 52 kDa; validate the pattern in relevant seminal fluid or cell lysates.
• In ELISA, this antibody can capture or detect native or recombinant SEMG1; optimization of coating and detection conditions is recommended.
• Since the antibody is confirmed to react with human samples, cross-reactivity with other species should be tested independently if required.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing platform, CamelBio supports diagnostic manufacturers and research institutions with reliable access to antibodies, antigens, and ancillary reagents. This SEMG1 polyclonal antibody is suitable for reproductive health and fertility research; CamelBio also offers matched antibody pairs, bulk-scale production, and customized sourcing for rare targets, streamlining your development workflow.