Anti-Semaphorin-4D/CD100 Rabbit Monoclonal Antibody for IF/ICC, FC, ELISA - Q92854

Core Product Specifications and Parameters

Biological Background: Semaphorin-4D Function and Localization

• Semaphorin-4D (also known as CD100, A8, BB18, GR3) is a single-pass type I membrane protein that exists as both membrane-bound and secreted forms. It belongs to the semaphorin family and contains an immunoglobulin domain.
• Acts as a cell surface receptor for plexins PLXNB1 and PLXNB2, mediating cell-cell signaling important for neurogenesis and immune regulation. Related references: PMID:20877282
• In the nervous system, it modulates neurite outgrowth and arborization via RHOA activation through PLXNB1, and promotes the development of inhibitory GABAergic synapses. Related references: PMID:19788569
• Drives cerebellar granule cell migration and endothelial cell migration via SRC-PTK2B-AKT signaling. Related references: PMID:16055703
• In the immune system, it induces B-cell aggregation and enhances their viability in vitro, highlighting a role in lymphocyte biology. Related references: PMID:8876214
• Highly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus, with lower expression in brain, kidney, and other tissues; not detected in colon and liver.
• Post-translationally modified by glycosylation and phosphorylation; subject to alternative splicing. Subcellularly localizes to the cell membrane and is also secreted.

Experimental Guidance and Technical Tips

• The immunogen covers the entire extracellular domain (aa 22-734), suggesting the antibody likely detects extracellular epitopes. For flow cytometry, consider using live, non-permeabilized cells to verify surface staining.
• For immunofluorescence/immunocytochemistry (IF/ICC), membrane localization requires proper permeabilization with detergents like Triton X-100 at low concentrations. Validate staining patterns in known Semaphorin-4D-expressing cell lines.
• In ELISA, the antibody may be used as a capture or detection reagent; cross-reactivity with other semaphorin family members should be assessed if working with complex biological samples.
• Due to alternative splicing and glycosylation, the observed protein weight on western blot may vary; if using this antibody for western blot, validate with appropriate controls and consider that the immunogen is recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline procurement of IVD raw materials, including this rabbit monoclonal antibody targeting Semaphorin-4D for neuroscience and immunology applications. Our portfolio encompasses validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development and production needs. For rare targets or custom sourcing requirements, CamelBio's global sourcing network offers tailored solutions to bridge your R&D pipeline from concept to clinic efficiently.