Anti-Semaphorin 4D / CD100 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q92854

Core Product Specifications and Parameters

Biological Background: Semaphorin-4D / CD100 Function and Localization

• Semaphorin-4D (SEMA4D, CD100) is a member of the class IV semaphorin family, characterized by an immunoglobulin-like domain and a conserved sema domain, existing as both membrane-bound and secreted forms.
• Serves as a cell surface receptor for plexin B1 (PLXNB1) and B2 (PLXNB2), transducing signals that regulate cell-cell communication, cytoskeletal dynamics, and cell migration. Related references: PMID:20877282
• Acts as an immunomodulator by inducing B-cell aggregation and promoting B-cell viability, underscoring its involvement in adaptive immune responses. Related references: PMID:8876214
• Promotes inhibitory synapse development and modulates neurite outgrowth complexity in hippocampal neurons via PLXNB1-dependent RHOA activation. Related references: PMID:19788569
• Drives endothelial cell migration through the PTK2B/PYK2-SRC-AKT pathway and facilitates cerebellar granule cell migration. Related references: PMID:16055703
• Predominantly found on the cell membrane and also present in secreted forms, allowing both juxtacrine and paracrine signaling.
• Highly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus; lower levels in brain, kidney, heart, placenta, and other tissues, but absent in colon and liver.
• Contains disulfide bonds and is N-glycosylated, with a calculated molecular weight of approximately 96 kDa; known to be phosphorylated, potentially regulating its signaling activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 100–200 of human Semaphorin-4D, covering the N-terminal immunoglobulin-like domain critical for ligand-receptor interactions.
• As a rabbit monoclonal antibody, expect high lot-to-lot consistency and strong affinity for applications like Western blot (WB), immunohistochemistry (IHC-P), and ELISA.
• For WB, the predicted molecular weight is 96 kDa; however, confirm band specificity using appropriate positive and negative controls (e.g., human spleen or lymphocyte lysates) due to potential glycosylation shifts.
• In IHC-P, human tissue sections with known expression (e.g., spleen, thymus) should be used for optimization; antigen retrieval conditions may need adjusting.
• This antibody is validated only for human cross-reactivity; do not assume reactivity with mouse or rat orthologs without verification.
• Consider using this antibody in functional studies exploring semaphorin-plexin signaling in immune cell aggregation, neuronal synapse formation, or cancer cell migration assays.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like Semaphorin-4D/CD100 with implications in immunology and neurology, our supply capabilities cover everything from rare target raw materials to custom production, streamlining your transition from concept to clinic.