Anti-Semaphorin 3A Rabbit Polyclonal Antibody for WB, ELISA - Q14563

Core Product Specifications and Parameters

Biological Background: Semaphorin 3A Function and Localization

• Semaphorin-3A (Sema III) is a secreted member of the semaphorin family of axon guidance molecules, encoded by the SEMA3A gene in humans.
• It functions as a chemorepellent that induces growth cone collapse and paralysis in neurons, binding to the neuropilin-1/plexin-1 receptor complex.
• Plays an essential role in the development of the olfactory system, guiding axonal projections during embryonic and postnatal stages.
• Contributes to the neuronal regulation of puberty onset, linking neurodevelopment with reproductive function.
• Subcellular localization is primarily secreted into the extracellular space, allowing it to act over distances to exert its guidance effects.
• Highly expressed in the dorsal root ganglia, consistent with its role in sensory neuron pathfinding and development.
• Post-translational modifications include disulfide bonds and glycosylation, which are important for protein stability and function.
• Mutations in SEMA3A are associated with Kallmann syndrome and hypogonadotropic hypogonadism, as indicated by disease variant annotations.

Experimental Guidance and Technical Tips

• The immunogen peptide is derived from amino acids 300-400 of human Semaphorin 3A, a region likely to be immunogenic; consider this for specificity assessments.
• For western blotting, expect a band near 89 kDa; include neuronal tissue lysates (e.g., dorsal root ganglia) as positive controls.
• When using with mouse or rat samples, validate antibody reactivity due to potential interspecies sequence variations.
• Optimize dilution and incubation conditions for each application; ELISA may require pre-absorption with the immunogen peptide to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, offering one-stop access to high-quality antibodies, reagents, and technical services from concept to clinic. Beyond this anti-Semaphorin 3A antibody, we provide validated antibody pairs, optimized monoclones and polyclones, and bulk ancillary reagents to support your neurodevelopment or diagnostic initiatives. For rare or custom targets, our expert sourcing ensures you receive the right materials efficiently, accelerating your research and product development timelines.