Anti-Semaphorin 3A Rabbit Monoclonal Antibody for WB, ELISA - Q14563

Core Product Specifications and Parameters

Biological Background: Semaphorin 3A Function and Localization

• Semaphorin-3A (Sema III) is a secreted protein and a member of the semaphorin family, characterized by an immunoglobulin domain and glycosylation.
• It plays a critical role in neuronal development, particularly in the olfactory system and in the neuronal control of puberty.
• Functions as a chemorepellent by inducing collapse and paralysis of neuronal growth cones, guiding specific growth cones via a motility-inhibiting mechanism.
• Acts as a ligand for the neuropilin-1/plexin-1 receptor complex, mediating downstream signaling cascades.
• Expressed in the dorsal root ganglia during development, with tissue-specific roles in sensory neuron guidance.
• Annotated as a developmental protein involved in neurogenesis and differentiation, with disulfide bonds contributing to its structural stability.
• Genetic variants in SEMA3A are associated with Kallmann syndrome and hypogonadotropic hypogonadism, linking it to human disease phenotypes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 582–771 of human Semaphorin 3A, covering the C-terminal region; consider epitope accessibility in your assay design.
• For Western blotting, an expected band at approximately 89 kDa should be observed. Validate detection in relevant sample systems, such as neuronal tissues or cell lines known to express SEMA3A.
• In ELISA applications, assess coating and detection conditions empirically; the antibody may be used as a capture or detection reagent depending on the experimental setup.
• The antibody cross-reacts with mouse and rat SEMA3A; verify performance in your specific model system, as sequence homology may affect sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including this Semaphorin 3A rabbit monoclonal antibody for neurobiology and developmental research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target sourcing—enabling you to build complete assay solutions from concept to clinic. Let our expertise bridge your procurement gaps and accelerate your project timelines.