Anti-SEMA7A Polyclonal Antibody for WB, ELISA - O75326

Core Product Specifications and Parameters

Biological Background: Semaphorin-7A Function and Localization

• Encodes Semaphorin-7A, a GPI-anchored membrane glycoprotein belonging to the semaphorin family; also known as CDw108 and JMH blood group antigen.
• Functions in integrin-mediated signaling, promoting focal adhesion complex formation and activation of PTK2/FAK1, leading to MAPK1/MAPK3 phosphorylation.
• Regulates immune responses by inducing pro-inflammatory cytokine production in monocytes and macrophages, and modulating T-cell-mediated inflammation.
• Supports neuronal development by promoting axon growth in the embryonic olfactory bulb.
• Facilitates melanocyte attachment, spreading, and dendrite outgrowth.
• Subcellular localization: anchored to the cell membrane via a GPI moiety; expressed in skin keratinocytes, endothelial cells, and widely in tissues such as placenta, testis, ovary, spleen, brain, spinal cord, lung, heart, adrenal gland, lymph nodes, thymus, intestine, and kidney.
• Contains an immunoglobulin domain and sema domain; subject to alternative splicing and disulfide bond formation.

Experimental Guidance and Technical Tips

• For WB, the expected band is approximately 75 kDa; consider using appropriate membrane blocking and antibody dilution validated in your sample system.
• The immunogen spans the extracellular sema domain (aa 45-280); thus, the antibody may detect both full-length GPI-anchored and shed soluble SEMA7A forms.
• Since SEMA7A is membrane-associated, optimize protein extraction with membrane fraction enrichment protocols for enhanced detection.
• For ELISA, validate the antibody with recombinant SEMA7A protein or appropriate native samples to establish optimal working conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing diagnostic manufacturers and labs with streamlined access to validated polyclonal antibodies like this anti-SEMA7A pAb. Our offerings span optimized antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing — all supported by technical and supply-chain expertise. From immunology and inflammation research to neurobiology studies, CamelBio bridges your development needs from concept to clinic.