Anti-SEMA4F Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95754

Core Product Specifications and Parameters

Biological Background: SEMA4F Function and Localization

• SEMA4F, identified as Semaphorin-4F (also known as Sema M or Sema W), is a member of the semaphorin family, which plays key roles in neuronal development and immune responses.
• It functions as a probable cell surface receptor involved in regulating the migration of oligodendroglial precursor cells and may also influence their differentiation (By similarity).
• Exhibits growth cone collapse activity against retinal ganglion-cell axons, indicating a role in axon guidance and neural circuit formation (By similarity).
• Predominantly localizes to the cell membrane, with additional presence in the postsynaptic density, perikaryon, and dendritic projections, supporting its involvement in synaptic function.
• Contains structural features typical of semaphorins, including an immunoglobulin domain, a transmembrane helix, and disulfide bonds, classifying it as a transmembrane receptor.
• Predicted to undergo alternative splicing, glycosylation, and phosphorylation, offering potential regulatory mechanisms for its activity.
• Classified under neurogenesis and developmental protein keywords, with implications in synaptic organization and neuronal differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280–420 within the extracellular domain of human SEMA4F; consider that this region may be conserved across species, as indicated by cross-reactivity with mouse and rat.
• For Western blot, the predicted molecular weight is approximately 84 kDa, but glycosylation may result in a higher apparent molecular mass; validate in the relevant sample system.
• In immunohistochemistry (paraffin-embedded), antigen retrieval conditions should be optimized for neuronal tissues, given SEMA4F's localization in dendrites and synapses.
• For ELISA applications, confirm specificity using appropriate positive and negative controls, as polyclonal antibodies may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting researchers and diagnostic manufacturers from concept to clinic. This anti-SEMA4F polyclonal antibody addresses targets in neurogenesis and cell migration research areas. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and assist in sourcing rare-target raw materials to streamline your development workflow.