Anti-SEMA3C Polyclonal Antibody for WB, IF/ICC, ELISA - Q99985

Core Product Specifications and Parameters

Biological Background: SEMA3C Function and Localization

• SEMA3C encodes semaphorin-3C, also known as Semaphorin-E (Sema E), a member of the semaphorin family of secreted and membrane-associated proteins involved in axon guidance and developmental processes.
• It plays a critical role in cardiovascular development during embryogenesis, functioning as a chemoattractant for growing axons and participating in neural network formation.
• The protein is secreted and contains an immunoglobulin (Ig) domain, characteristic of the semaphorin family, which mediates interactions with plexin receptors.
• Tissue expression is prominent in the heart, skeletal muscle, colon, small intestine, ovary, testis, and prostate, with lower ubiquitous expression elsewhere, including the brain.
• Post-translational modifications include disulfide bonds and N-linked glycosylation, which may influence protein stability and function. Alternative splicing generates multiple isoforms.
• Keywords from UniProt highlight its involvement in developmental biology, neurogenesis, and differentiation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21–330 of human SEMA3C (NP_006370.1), spanning the N-terminal region including the semaphorin domain. This region is expected to detect full-length SEMA3C and may also recognize isoforms containing this portion.
• For Western blot analysis, a prominent band near 85 kDa is anticipated; however, glycosylation can cause a molecular weight shift. Validate band specificity using positive controls from tissues with high SEMA3C expression (e.g., heart, skeletal muscle).
• In immunofluorescence/ICC experiments, since SEMA3C is a secreted protein, detection may require permeabilization and careful fixation to visualize intracellular trafficking or extracellular matrix association. Use appropriate negative controls.
• For ELISA applications, the recombinant immunogen can be employed as a coating antigen to confirm antibody binding; consider cross-reactivity with mouse SEMA3C as supported by the reported cross-reactivity data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B platform for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality antibodies, including polyclonal antibodies like this anti-SEMA3C product. Specializing in developmental biology and neuroscience targets, we offer a wide range of validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Whether you need ready-to-use reagents or tailored procurement solutions, CamelBio accelerates your path from concept to clinic with reliable supply chain support.