Anti-SEMA3B Rabbit Polyclonal Antibody for WB and ELISA - Q13214

Core Product Specifications and Parameters

Biological Background: Semaphorin-3B Function and Localization

• Semaphorin-3B (SEMA3B), also known as Sema A(V) or Semaphorin-V, is a secreted glycoprotein encoded by the SEMA3B gene. It belongs to the semaphorin family and contains immunoglobulin domains and disulfide bonds.
• The primary function of SEMA3B is to inhibit axonal extension, acting as a local repulsive signal that guides growing axons during neural development.
• Subcellularly, the protein is secreted into the extracellular space and is also localized to the endoplasmic reticulum.
• SEMA3B is expressed abundantly but differentially in a variety of both neural and nonneural tissues, indicating broad regulatory roles.
• Post-translational modifications include glycosylation and the formation of disulfide bonds, which are critical for its structural integrity and function.
• Alternative splicing generates multiple isoforms, potentially expanding its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 569–748) of human SEMA3B. Consider epitope accessibility when choosing between native and denaturing conditions.
• For Western blot (WB), the expected band size is approximately 83 kDa. Use relevant positive controls such as human or mouse neural tissue lysates.
• For ELISA applications, validate assay conditions with recombinant SEMA3B protein or known positive samples to ensure specificity.
• Cross-reactivity with human and mouse SEMA3B is indicated; if using other species, verify sequence homology of the target region.
• As a secreted protein, sample preparation may require consideration of secretion pathways or enrichment strategies.
• Being a polyclonal antibody, it may recognize multiple forms of SEMA3B, including isoforms or cleavage products; confirm target identity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a seamless pathway from concept to clinic for diagnostic manufacturers, laboratories, and research institutes. Our SEMA3B antibody supports research in neural development and axon guidance, complementing a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Let us streamline your procurement process and help accelerate your assay development.