Anti-SELENBP1 Rabbit Polyclonal Antibody for WB, ELISA - Q13228

Core Product Specifications and Parameters

Biological Background: SELENBP1 Function and Localization

• Encodes Methanethiol oxidase, also known as 56 kDa selenium-binding protein (SBP56), which binds selenium and may sense reactive xenobiotics in the cytoplasm.
• Functions as a methanethiol oxidase, converting methanethiol produced by gut bacteria, with implications in sulfur metabolism. Related references: PMID:29255262
• Subcellularly, localizes to the nucleus, cytoplasm, and cytosol, and is associated with membranes, suggesting roles in intracellular transport (by similarity).
• Tissue distribution is widespread, with high expression in liver, lung, colon, prostate, kidney, and pancreas; also detected in brain neurons and glia.
• Down-regulated in multiple cancers, including lung adenocarcinoma, colorectal carcinoma, and ovarian cancer, indicating potential as a biomarker.
• Up-regulated two-fold in brain and blood from schizophrenia patients, linking it to neuropsychiatric disorders.
• Post-translational modifications include acetylation and phosphorylation; the protein undergoes alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 52 kDa. Reduce samples and boil prior to loading. Validate detection in human and mouse lysates as the antibody cross-reacts with both species.
• In ELISA, the polyclonal antibody may serve as a capture or detection reagent. When developing a sandwich assay, consider pairing with a monoclonal antibody targeting a distinct epitope for optimal sensitivity.
• The immunogen corresponds to amino acids 243–472, an internal region. Some isoforms generated by alternative splicing may lack this sequence; confirm isoform expression in your model system.
• Because SELENBP1 binds selenium, sample preparation conditions (e.g., use of strong reducing agents) could affect epitope accessibility. Optimize lysis and denaturation steps empirically.
• Lot-specific performance may vary for polyclonal antibodies. Include appropriate positive and negative controls, and consider routine bridging studies when changing lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and researchers as a comprehensive partner for IVD raw-material procurement, covering every stage from concept to clinic. Our catalog provides access to validated antibody pairs, bulk ancillary reagents, and rare-target raw materials. This SELENBP1 antibody supports projects in cancer biomarker research and metabolic disease studies, and we can assist in scaling up supply or sourcing complementary reagents to accelerate your development.