Anti-SELE Polyclonal Antibody for WB and ELISA - P16581

Core Product Specifications and Parameters

Biological Background: E-selectin Function and Localization

• E-selectin (SELE), also known as CD62E, ELAM-1, and LECAM2, is a cell-surface glycoprotein belonging to the selectin family of adhesion molecules.
• It mediates the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1, playing a central role in immunoadhesion and leukocyte recruitment during inflammation.
• SELE may also contribute to capillary morphogenesis, suggesting a role in angiogenesis and vascular remodeling.
• The protein is localized to the cell membrane and is characterized by an extracellular region containing a C-type lectin domain, an EGF-like domain, and multiple sushi (SCR) repeats, followed by a transmembrane helix and a short cytoplasmic tail.
• Key structural features include calcium-dependent carbohydrate recognition via the lectin domain, consensus repeats stabilized by disulfide bonds, and metal-binding sites.
• Post-translational modifications include disulfide bond formation and glycosylation, consistent with its function as a glycoprotein adhesion receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 22-230), which includes the lectin and EGF-like domains; this region is critical for ligand binding and may ensure detection of functional epitopes.
• For WB, the predicted molecular weight is 67kDa, but note that glycosylation may cause a shift; consider using appropriate positive controls (e.g., cytokine-activated endothelial cell lysates).
• In ELISA, the antibody may be used for capture or detection; validate in the relevant sample system with recombinant SELE or activated endothelial cells.
• As a polyclonal antibody, it recognizes multiple epitopes, offering robust signal but potential for cross-reactivity; always include proper controls.
• Cross-reactivity with mouse and rat has been reported, making it suitable for comparative studies across species; confirm reactivity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our anti-SELE polyclonal antibody exemplifies our commitment to supplying validated reagents for immunoadhesion and inflammation research. Beyond this product, we offer extensively validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development pipeline.