Anti-Secretagogin (SCGN) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O76038

Core Product Specifications and Parameters

Biological Background: Secretagogin (SCGN) Function and Localization

• Secretagogin (SCGN) is a calcium-binding protein belonging to the EF-hand superfamily, characterized by multiple helix-loop-helix motifs that mediate calcium-dependent conformational changes.
• It is highly expressed in pancreatic islets of Langerhans, particularly in beta cells, and to a lesser extent in the gastrointestinal tract, adrenal medulla/cortex, and thyroid C-cells, indicating a role in endocrine and neuroendocrine secretion.
• In the brain, SCGN expression is restricted to specific neuronal populations, including cerebellar stellate and basket cells, thalamic, hypothalamic, and neocortical neurons, as well as the anterior pituitary.
• Subcellularly, SCGN is found in the cytoplasm and can be secreted; it also associates with the membrane of secretory vesicles, consistent with its implication in regulated exocytosis.
• The protein contains consensus EF-hand calcium-binding domains and is annotated with keywords including Calcium, Metal-binding, Repeat, and Secreted, reflecting its functional classification.
• Tissue-specific expression data and proteomic identification confirm SCGN as a reliable marker for neuroendocrine differentiation.

Experimental Guidance and Technical Tips

• The immunogen design spans amino acids 1–276 of human SCGN (near full-length), suggesting that the antibody may detect both denatured and native conformations, supporting applications such as WB, IHC-P, and IF/ICC.
• For Western blot, note that SCGN has a predicted molecular weight of 32 kDa; consider using appropriate positive controls such as pancreatic islet lysates or neuroendocrine cell lines.
• Given the reported cross-reactivity with mouse and rat, this antibody is suitable for studies in rodent models; however, always validate in the relevant species and tissue system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody dilution, and include negative controls (e.g., tissues with low or absent SCGN expression) to confirm specificity.
• Since SCGN is a secreted and cytoplasmic protein, permeabilization is required for intracellular detection in IF; also consider the possibility of extracellular staining in secretory granules.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents for diagnostic development. This anti-Secretagogin pAb can be integrated into immunoassay platforms targeting calcium-binding proteins in metabolic and neurological disorders. Beyond routine catalog supply, CamelBio specializes in procuring rare-target reagents and providing technical support from concept to clinic.