Anti-Secretagogin (SCGN) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O76038

Core Product Specifications and Parameters

Biological Background: Secretagogin Function and Localization

• Secretagogin (SCGN), encoded by the SCGN gene, is a calcium-binding protein of the EF-hand superfamily.
• It contains EF-hand repeats and has a molecular weight of approximately 32 kDa (UniProt: 32040 Da).
• SCGN is predominantly localized in the cytoplasm, and can also be secreted and associated with secretory vesicle membranes.
• High expression is observed in pancreatic islets of Langerhans, with lower levels in the gastrointestinal tract, adrenal gland, and thyroid C-cells.
• In the brain, SCGN is restricted to specific neuronal subtypes, including cerebellar stellate and basket cells, thalamus, hypothalamus, and some neocortical neurons.
• Keywords from UniProt suggest its involvement in calcium signaling and metal-binding processes.

Experimental Guidance and Technical Tips

• When performing Western blot, expect a band near 32 kDa corresponding to the monomeric form; it is advisable to include appropriate controls to confirm specificity.
• For immunohistochemistry, consider optimizing antigen retrieval conditions and antibody dilutions in the relevant tissue type.
• The immunogen region (aa 177-276) is conserved across human, mouse, and rat, supporting the antibody's cross-reactivity.
• ELISA applications may require coating the peptide directly or using a sandwich format; validate in the sample matrix of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, technical services, and consulting to support diagnostic manufacturers and research institutions. This anti-Secretagogin antibody targets a key calcium-binding protein relevant to neuroendocrine function and pancreatic studies. Beyond validated antibody pairs and optimized monoclonal/polyclonal antibodies, we provide bulk ancillary reagents and can assist in sourcing rare-target raw materials to streamline your development pipeline.