Anti-SECISBP2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96T21

Core Product Specifications and Parameters

Biological Background: SECISBP2 Function and Localization

• SECISBP2 (also known as SBP2) is the selenocysteine insertion sequence-binding protein 2, a critical RNA-binding protein required for the synthesis of selenoproteins.
• It specifically binds the SECIS element in the 3'-UTR of selenoprotein mRNAs, enabling the incorporation of the rare amino acid selenocysteine at UGA codons through a coordinated mechanism with the elongation factor EEFSEC. Related references: PMID:35709277
• The protein localizes to both the nucleus and mitochondrion, indicating its functional roles in different cellular compartments.
• Expression is notably high in testis, suggesting tissue‑specific regulation of selenoprotein synthesis.
• Alternative splicing generates multiple isoforms, and mutations in SECISBP2 are associated with disease variants.
• It contains an N‑terminal transit peptide and is classified under protein biosynthesis and proteomics identification keywords.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fragment (aa 585‑854) of human SECISBP2, a region unique to this protein and conserved across species, supporting its cross‑reactivity with mouse and rat.
• For Western blotting, expect a band at approximately 95 kDa; lysates from testis or other selenoprotein‑rich tissues may provide the strongest signal.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), consider testing both nuclear and mitochondrial staining patterns.
• ELISA applications may require further optimization of coating antigen and detection conditions.
• Always include appropriate positive and negative controls to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials. This anti‑SECISBP2 polyclonal antibody is an example of our offerings tailored for selenoprotein and translation research. Through our integrated supply network, we provide access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing to accelerate your assay development from concept to clinic.