Anti-SEC63 Rabbit Polyclonal Antibody for WB and ELISA - Q9UGP8

Core Product Specifications and Parameters

Biological Background: SEC63 Function and Localization

• SEC63 (also known as ERdj2, DNAJC23, and PCLD2) is a DnaJ homolog subfamily C member and an ER-resident transmembrane chaperone.
• It mediates cotranslational and post-translational protein translocation into the ER. Related references: PMID:22375059 PMID:29719251
• SEC63 recognizes precursors with short, apolar signal peptides and cooperates with SEC62 and HSPA5/BiP to target them to the SEC61 translocon. Related references: PMID:29719251
• It is required for efficient Polycystin-1 biogenesis and trafficking to cilia.
• Localized at the ER membrane; widely expressed with highest levels in liver.
• As a phosphoprotein with coiled-coil and transmembrane domains, SEC63 is implicated in protein transport and polycystic liver disease (aliased PCLD2).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 595-720) of human SEC63, which is likely to be accessible and specific.
• For Western blot (WB), a band at ~88 kDa is expected. Liver tissue lysates are recommended as positive control due to high SEC63 expression.
• This polyclonal antibody is suitable for ELISA; appropriate dilution and blocking conditions should be optimized empirically.
• Since SEC63 is conserved across species, the reported cross-reactivity with mouse and rat suggests utility in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material partner, CamelBio offers high-quality antibodies like this anti-SEC63 polyclonal antibody to support research into ER translocation mechanisms and liver disease. Our portfolio includes validated antibody pairs, recombinant proteins, and ancillary reagents, with customized sourcing for rare targets to accelerate your assay development from concept to clinic.