Anti-SEC62 Polyclonal Antibody for WB, ELISA - Q99442

Core Product Specifications and Parameters

Biological Background: SEC62 Function and Localization

• SEC62 (translocation protein SEC62, also known as TP-1/hTP-1, Dtrp1, TLOC1) is a multi-pass membrane protein that mediates post-translational transport of precursor polypeptides across the endoplasmic reticulum (ER).
• It acts as a targeting receptor for small presecretory proteins containing short and apolar signal peptides, properly positioning them into the SEC61 channel-forming translocon complex to trigger translocation into the ER lumen.
• SEC62 is localized to the endoplasmic reticulum membrane, consistent with its role in protein translocation.
• It belongs to a conserved translocation machinery and is predicted to contain multiple transmembrane helices.
• The protein undergoes phosphorylation and is part of the proteomics-identified reference proteome.
• Structural information (3D-structure) is available, indicating its functional domains and interactions.
• No tissue-specific expression data is available in this record, but its fundamental role in ER translocation suggests ubiquitous expression.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody is a recombinant fragment corresponding to amino acids 258-399 of human SEC62, which lies within the C‑terminal region of the protein. This region may provide good exposure for antibody binding.
• In Western blot (WB) applications, the antibody detects a band around 46 kDa (predicted molecular weight of the full‑length protein is approximately 46 kDa). Validate in the relevant sample system, such as lysates from human, mouse, or rat cell lines.
• For ELISA, consider using the antibody as a detection reagent after confirming its performance with recombinant SEC62 protein or cell extracts.
• Cross‑reactivity with mouse and rat SEC62 is reported, but sequence alignment should be checked if working with other species; the epitope may not be fully conserved.
• As with any polyclonal antibody, batch‑to‑batch variability can occur; critical applications may require re‑validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For your research on ER translocation and protein transport involving SEC62, CamelBio can support your project with high-quality raw materials and tailored supply solutions.