Anti-SEC61B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P60468

Core Product Specifications and Parameters

Biological Background: SEC61B Function and Localization

• SEC61B encodes the beta subunit of the SEC61 translocon complex, a central component of the endoplasmic reticulum (ER) protein translocation machinery. Related references: PMID:12475939
• The SEC61 complex forms a ribosome receptor and a gated pore in the ER membrane, enabling cotranslational import of signal peptide-containing precursor polypeptides. Related references: PMID:12475939
• It also mediates ER membrane insertion of transmembrane proteins, working in concert with the multi-pass translocon (MPT) complex for multi-spanning membrane proteins. Related references: PMID:32820719, PMID:36261522
• SEC61B cooperates with the translocating protein TRAM1 to facilitate nascent protein import into the ER. Related references: PMID:19121997
• Subcellularly, SEC61B is an integral component of the endoplasmic reticulum membrane.
• The protein undergoes several post-translational modifications, including acetylation, palmitoylation, and phosphorylation, which may regulate its activity or stability.
• As a key player in protein translocation, SEC61B is associated with processes such as protein transport, translocation, and ER membrane organization.

Experimental Guidance and Technical Tips

• For Western Blot (WB), the predicted molecular weight of SEC61B is approximately 10 kDa. Use a 12-15% SDS-PAGE gel and optimize transfer to a PVDF membrane with 0.2 µm pore size for small proteins. Include appropriate positive controls (e.g., HeLa, HEK293, or tissue lysates expressing SEC61B) and validate antibody specificity with a blocking peptide or knockout sample.
• For Immunofluorescence/Immunocytochemistry (IF/ICC), fixation with 4% PFA followed by permeabilization with 0.1% Triton X-100 is recommended. Test antibody concentration in the range of 1:50–1:200 and confirm localization at the ER. Co-staining with an ER marker (e.g., calnexin or PDI) can help verify specificity.
• For ELISA, the antibody can be evaluated as a capture or detection reagent. Titrate the antibody to determine the optimal working concentration, and validate with a recombinant SEC61B protein or cell lysate. Ensure cross-reactivity testing with mouse and rat samples to confirm performance.
• As a polyclonal antibody, lot-to-lot consistency should be monitored. Consider aliquoting upon receipt and performing a pilot study for each new lot to maintain experimental reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering comprehensive sourcing solutions from early-stage research to clinical application. This anti-SEC61B polyclonal antibody supports investigations into ER protein translocation and membrane insertion, areas critical to understanding cellular homeostasis and disease. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring you have reliable materials for your diagnostic and research workflows.