Anti-SEC61A1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P61619

Core Product Specifications and Parameters

Biological Background: SEC61A1 Function and Localization

• SEC61A1 encodes the alpha subunit isoform 1 of the Sec61 translocon complex, a highly conserved heterotrimeric channel in the endoplasmic reticulum (ER) membrane, essential for secretory and membrane protein biogenesis.
• As a core component of the SEC61 channel, it mediates cotranslational translocation of nascent polypeptides by functioning as both a ribosome receptor and a gated pore in the ER membrane. Related references: PMID:12475939, PMID:22375059, PMID:28782633, PMID:29719251
• In cooperation with SEC62, SEC63, and HSPA5/BiP, SEC61A1 facilitates post-translational transport of small presecretory proteins across the ER membrane. Related references: PMID:22375059, PMID:29719251
• The SEC61 channel is involved in ER membrane insertion of transmembrane proteins; it mediates insertion of the initial transmembrane segments, while subsequent regions are handled by the multi-pass translocon (MPT) complex. SEC61A1 also cooperates with TRAM1 for protein import. Related references: PMID:32820719, PMID:36261522, PMID:8616892
• SEC61A1 controls the passive efflux of calcium ions from the ER lumen to the cytosol, contributing to the maintenance of cellular calcium homeostasis. Related references: PMID:28782633
• Subcellularly localized to the ER membrane, consistent with its central role in secretory pathway protein translocation and processing.
• Expressed in proximal and distal tubules in kidney, SEC61A1 plays a critical role in nephrogenesis, particularly at the pronephros stage.
• Keywords associated with P61619 include 3D-structure, alternative splicing, chaperone, disease variant, protein transport, and translocation, reflecting its multifunctional role in cellular protein homeostasis.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is 52 kDa; validate the observed band in your specific cell or tissue lysate and consider appropriate loading controls.
• Immunofluorescence/ICC may require permeabilization to detect the ER membrane-localized SEC61A1; confirm the expected reticular staining pattern with organelle markers.
• This polyclonal antibody, generated against a recombinant protein fragment, may recognize multiple epitopes; take this into account when optimizing assay conditions and interpreting results.
• Cross-reactivity is expected with mouse and rat SEC61A1 based on immunogen design, but we recommend validating performance in the relevant species and sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like SEC61A1 involved in ER protein translocation, our one-stop platform streamlines procurement from discovery to clinical application. We also assist in sourcing rare and specialized raw materials to accelerate your assay development.