Anti-SEC61A1 Polyclonal Antibody for WB, ELISA - P61619

Core Product Specifications and Parameters

Biological Background: SEC61A1 Function and Localization

• SEC61A1 is a core subunit of the Sec61 translocon complex, serving as both a ribosome receptor and a gated pore for cotranslational translocation of nascent polypeptides across the ER membrane. Related references: PMID:12475939, PMID:22375059, PMID:28782633, PMID:29719251
• Together with SEC62, SEC63, and HSPA5/BiP, SEC61A1 may also mediate post‑translational import of small presecretory proteins. Related references: PMID:22375059, PMID:29719251
• It plays a role in ER membrane insertion of transmembrane proteins; initial transmembrane segments are inserted via SEC61, while later segments may use the multi‑pass translocon (MPT) complex. Related references: PMID:32820719, PMID:36261522
• SEC61A1 cooperates with TRAM1 to import nascent proteins into the ER. Related references: PMID:8616892
• Controls passive efflux of calcium ions from the ER lumen to the cytosol, contributing to cellular calcium homeostasis. Related references: PMID:28782633
• Critical for nephrogenesis, particularly at the pronephros stage (by similarity).
• The protein resides in the endoplasmic reticulum membrane and contains multiple transmembrane helices.
• At the protein level, it is expressed in proximal and distal tubules of the kidney.
• Alternative splicing generates multiple isoforms; gene defects are linked to diseases such as ADTKD5.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 310‑420) of human SEC61A1, a cytosolic segment likely accessible for antibody binding under denaturing (WB) and native (ELISA) conditions.
• For Western blot, expect a band near 52 kDa. Validate specificity by comparing signals in SEC61A1‑expressing cell lines or tissues (e.g., kidney).
• Given high sequence homology, cross‑reactivity with mouse and rat is supported; verify performance in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research laboratories by providing a comprehensive range of raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. For targets like SEC61A1—central to ER protein translocation and kidney development—we offer bulk sourcing and customization options to streamline your assay development. Partner with us to bridge the gap from concept to clinic with reliable supply and expert technical support.