Anti-SEC24D Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O94855

Core Product Specifications and Parameters

Biological Background: SEC24D Function and Localization

• SEC24D is a component of the COPII coat complex, essential for the formation of ER-to-Golgi transport vesicles. It facilitates membrane deformation and cargo selection for vesicle budding. (PubMed:17499046, PubMed:18843296, PubMed:20427317) Related references: PMID:17499046 PMID:18843296 PMID:20427317

• Within the COPII complex, SEC24D plays a central role in cargo recognition, and its specificity may differ from that of SEC24A and SEC24B when paired with SEC24C. Related references: PMID:17499046 PMID:18843296 PMID:20427317

• SEC24D specifically packages GPI-anchored proteins by interacting with the cargo receptor TMED10. Related references: PMID:20427317

• It also recognizes IxM motif-containing SNAREs, such as GOSR2 and STX5. Related references: PMID:18843296

• Subcellular localization: COPII-coated vesicle membrane, endoplasmic reticulum membrane, and cytosol.

• Ubiquitously expressed, with higher levels in placenta, pancreas, heart, and liver.

• Annotated with keywords: metal-binding (zinc), phosphoprotein; genetic variants linked to craniosynostosis and osteogenesis imperfecta.

Experimental Guidance and Technical Tips

• This polyclonal antibody is generated against a recombinant fragment (aa 830-1000) of human SEC24D; validate specificity in your target species (human, mouse, rat) as cross-reactivity is expected.
• For WB, the predicted molecular weight is ~113 kDa; consider using appropriate gel percentage and transfer conditions.
• For IF/ICC, optimize fixation/permeabilization protocols; SEC24D localizes to ER and COPII vesicles, so use appropriate organelle markers.
• ELISA may be used for quantifying SEC24D, but ensure the antibody is suitable for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material partner, providing validated antibodies and ancillary reagents to support your research and diagnostic development. This SEC24D polyclonal antibody aids in studying COPII-mediated ER-Golgi trafficking, and we can supply optimized antibody pairs, bulk reagents, and rare target sourcing. Contact us to streamline your supply chain from concept to clinic.