Anti-SEC24D Monoclonal Antibody for WB, ELISA - O94855

Core Product Specifications and Parameters

Biological Background: SEC24D Function and Localization

• SEC24D is a core component of the COPII coat complex, driving ER membrane deformation into transport vesicles and selecting cargo molecules for ER-to-Golgi transport. Related references: PMID:17499046, PMID:18843296, PMID:20427317
• It exhibits cargo-recognition specificity: together with SEC24C, SEC24D may preferentially package GPI-anchored proteins via the cargo receptor TMED10 and IxM motif-containing SNAREs such as GOSR2 and STX5. Related references: PMID:20427317, PMID:18843296
• Subcellularly, SEC24D localizes to COPII-coated vesicle membranes, the endoplasmic reticulum membrane, and the cytosol.
• Expression is ubiquitous, with elevated levels found in placenta, pancreas, heart, and liver.
• The protein contains zinc-binding and phosphorylation sites, and mutations are associated with craniosynostosis and osteogenesis imperfecta.
• Multiple isoforms are produced by alternative splicing, expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–100), which is likely conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, the predicted molecular weight is ~113 kDa; use appropriate molecular weight markers and optimize transfer/blocking conditions for high-molecular-weight proteins.
• In ELISA applications, consider pairing with a detection antibody targeting a non-overlapping epitope for sandwich assays.
• Validate antibody specificity in your sample system using knockdown or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers a streamlined sourcing solution for IVD raw materials, including validated monoclonal antibodies like this anti-SEC24D clone for intracellular trafficking and organelle studies. Our portfolio extends to optimized antibody pairs, bulk ancillary reagents, and custom sourcing of rare targets, supporting every stage from concept to clinic.