Anti-SEC24C Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P53992

Core Product Specifications and Parameters

Biological Background: SEC24C Function and Localization

• SEC24C (SEC24-related protein C) is a component of the COPII coat complex, which drives vesicle formation from the endoplasmic reticulum (ER) for cargo transport to the Golgi apparatus. Related references: PMID:10214955
• It plays a key role in cargo selection, with a specificity profile distinct from SEC24A and SEC24B; together with SEC24D, it may preferentially package GPI-anchored proteins through the cargo receptor TMED10. Related references: PMID:17499046, PMID:18843296, PMID:20427317
• SEC24C specifically recognizes IxM motif-containing cargos, such as the SNARE proteins GOSR2 and STX5, facilitating their incorporation into COPII vesicles. Related references: PMID:18843296
• The protein localizes to the cytoplasmic side of the ER membrane, COPII-coated vesicle membranes, and the cytosol, consistent with its role in vesicle budding.
• Ubiquitously expressed across tissues, reflecting its fundamental function in secretory pathway trafficking.
• Post-translational modifications include phosphorylation, and the protein binds zinc, as inferred from its metalloprotein designation.

Experimental Guidance and Technical Tips

• For Western blot, validate the antibody in human cell lysates; an expected band around 118 kDa corresponds to full-length SEC24C. Membrane preparation or enrichment may improve signal.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions to visualize ER and COPII vesicle patterns; co-staining with organelle markers is recommended.
• For ELISA, the immunogen (C-terminal fragment) can be used as a positive control; assess performance with recombinant SEC24C or endogenous protein from cell extracts.
• Due to the polyclonal nature, lot-to-lot consistency should be verified using a characterized reference standard if quantitative applications are intended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to IVD raw materials, spanning validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-SEC24C product supports ER-Golgi transport and secretory pathway research, and CamelBio can assist with complementary reagents or custom sourcing for complete assay development workflows.