Anti-SEC23IP Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6Y8

Core Product Specifications and Parameters

Biological Background: SEC23IP Function and Localization

• SEC23IP (p125) is a SEC23-interacting protein that plays a pivotal role in the organization of endoplasmic reticulum (ER) exit sites.
• It specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P), and phosphatidylinositol 5-phosphate (PI(5)P), linking phospholipid signaling to COPII vesicle formation.
• Subcellularly, SEC23IP localizes to cytoplasmic COPII-coated vesicle membranes and the ER membrane.
• The protein undergoes phosphorylation and alternative splicing, resulting in multiple isoforms.
• It is ubiquitously expressed, with higher levels detected in heart, liver, and skeletal muscle.
• Keywords associated with SEC23IP include Alternative splicing, Cytoplasmic vesicle, Direct protein sequencing, Endoplasmic reticulum, Membrane, Phosphoprotein, Proteomics identification, and Reference proteome.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment (aa 600–940) of human SEC23IP; validate specificity in your experimental system.
• For western blotting of the 111 kDa protein, use appropriate gel acrylamide percentages and optimize transfer conditions.
• Cross-reactivity with mouse and rat is reported; confirm performance if using species other than human.
• In immunofluorescence applications, anticipate ER and COPII vesicle staining; consider co-localization with organelle markers.
• Be aware that alternative splicing may affect epitope recognition in certain SEC23IP isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibodies for intracellular trafficking targets like SEC23IP. Our offerings encompass polyclonal and monoclonal antibodies, matched antigen pairs, and bulk reagents, streamlining your assay development from early R&D to clinical application. Through our extensive sourcing network, we also facilitate access to rare and custom raw materials, ensuring your projects stay on track.