Anti-Sec23B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15437

Core Product Specifications and Parameters

Biological Background: Sec23B Function and Localization

• Sec23B is a protein transport protein, also known as SEC23-related protein B, encoded by the SEC23B gene. It is a core component of the coat protein complex II (COPII) that mediates vesicle budding from the endoplasmic reticulum (ER).
• Together with Sec24, Sec23B forms the inner layer of the COPII coat, promoting the physical deformation of the ER membrane into transport vesicles and the selective packaging of cargo molecules destined for the Golgi complex.
• Sec23B exhibits GTPase-activating protein (GAP) activity toward the small GTPase Sar1, which is critical for coat assembly and disassembly during vesicle formation.
• The protein is ubiquitously expressed across human tissues, underscoring its fundamental role in the secretory pathway and membrane biogenesis.
• At the subcellular level, Sec23B localizes to cytoplasmic vesicles, specifically COPII-coated vesicle membranes, the ER membrane, and the cytosol.
• Post-translational modifications include acetylation; mutations in SEC23B are associated with congenital dyserythropoietic anemia type II (CDAII), a hereditary hemolytic anemia characterized by ineffective erythropoiesis and bi-nucleated erythroblasts.
• Sec23B has a molecular weight of approximately 86 kDa and contains zinc-binding motifs, classifying it as a zinc metalloprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from residues 150–250 of human Sec23B, a region that is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting (WB), a band corresponding to the predicted molecular weight of 86 kDa should be detected. Consider testing a range of antibody dilutions (e.g., 1:500–1:2000) and validate with appropriate positive and negative controls in your sample system.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect a staining pattern consistent with the ER and COPII vesicle distribution. Co-localization studies with organelle markers (e.g., ER-Golgi intermediate compartment markers) can help confirm specificity.
• For ELISA, the antibody may be used for capture or detection. Perform checkerboard titrations to determine optimal coating and antibody concentrations.
• Since Sec23B is ubiquitously expressed, most cultured cell lines (e.g., HeLa, HEK293, NIH/3T3) should contain the target. Ensure that the lysis buffer and protocol are compatible with membrane-associated proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of antibodies, antigens, and ancillary reagents to diagnostic manufacturers, laboratories, and research institutes worldwide. This anti-Sec23B polyclonal antibody supports research into ER-Golgi transport, vesicle trafficking, and related disorders such as congenital dyserythropoietic anemia. Beyond individual products, CamelBio can assist in securing validated antibody pairs, bulk ancillary reagents, and custom reagents for rare targets, streamlining procurement from concept to clinic.