Anti-Sec23A Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q15436

Core Product Specifications and Parameters

Biological Background: Sec23A Function and Localization

• Sec23A encodes the protein transport protein Sec23A, also known as SEC23-related protein A, a core component of the coat protein complex II (COPII).
• It promotes the formation of transport vesicles from the endoplasmic reticulum (ER), playing a crucial role in cargo selection and membrane deformation during ER-to-Golgi trafficking.
• Functions in insulin-responsive translocation: required for the movement of GLUT4 (SLC2A4) glucose transporter to the plasma membrane upon insulin stimulation.
• Subcellular localization includes the COPII-coated vesicle membrane, ER membrane, and cytosol, reflecting its dynamic role in vesicle budding.
• Subject to post-translational modifications, including acetylation and phosphorylation, as identified by proteomics approaches.
• Ubiquitously expressed across human tissues, indicating its fundamental role in secretory pathway function.
• Annotated with the 'Disease variant' keyword, implying that genetic alterations in SEC23A are associated with human disorders.
• Multiple transcript variants are generated through alternative splicing, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment spanning amino acids 300–400 of human Sec23A; validate reactivity in your specific sample system and target species.
• For Western blot, expect a specific band near 86 kDa. Include a positive control lysate from cells or tissues known to express Sec23A, such as HeLa or liver.
• In immunofluorescence, Sec23A exhibits cytoplasmic and ER-like staining. Optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) for accurate localization.
• For ELISA applications, pair this monoclonal antibody with a suitable detection antibody and use a recombinant Sec23A standard curve to ensure assay linearity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, providing a one-stop platform for high-quality IVD raw materials. This anti-Sec23A antibody supports investigations into ER-Golgi transport, COPII vesicle biology, and insulin-regulated trafficking pathways. Beyond individual antibodies, CamelBio offers validated antibody pairs, recombinant antigens, bulk ancillary reagents, and specialized materials for rare targets—empowering you to accelerate assay development from concept to clinical application.