Anti-Sec23A Polyclonal Antibody for WB, ELISA - Q15436

Core Product Specifications and Parameters

Biological Background: Sec23A Function and Localization

• Sec23A, also known as SEC23-related protein A, is a core component of the coat protein complex II (COPII) that drives transport vesicle formation at the endoplasmic reticulum (ER).
• It participates in both the physical deformation of the ER membrane into vesicles and the selection of cargo molecules for delivery to the Golgi complex.
• Plays a role in insulin signaling by facilitating the translocation of the glucose transporter GLUT4 (SLC2A4) to the cell surface (by similarity).
• Localizes to the ER membrane, COPII-coated vesicle membranes, and the cytosol, reflecting its dynamic role in vesicular trafficking.
• Expressed ubiquitously in human tissues.
• Undergoes post-translational modifications such as acetylation and phosphorylation; contains zinc-finger motifs that may mediate protein–protein or protein–membrane interactions.
• Multiple isoforms are generated through alternative splicing.
• Mutations in SEC23A have been linked to disease (exact variant not detailed in current record).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-150) of human Sec23A; for western blot, a band of approximately 86 kDa is expected.
• For ELISA applications, plate coating with the full-length recombinant protein or cell lysates from relevant species may be suitable; assay conditions should be optimized empirically.
• Cross-reactivity with mouse and rat is stated; however, users should verify reactivity in their specific sample matrix, e.g., using positive and negative controls.
• As a polyclonal antibody, batch-to-batch consistency should be assessed for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by offering a comprehensive portfolio of raw materials, including validated Sec23A antibodies for ER-to-Golgi transport and COPII vesicle research. From optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, we support your assay development from concept to clinic. Contact us to explore tailored procurement solutions for your diagnostic needs.