Anti-SEC14L2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O76054

Core Product Specifications and Parameters

Biological Background: SEC14L2 Function and Localization

• SEC14-like protein 2 (SEC14L2), also designated alpha-tocopherol-associated protein (TAP, hTAP), supernatant protein factor (SPF), or squalene transfer protein, is encoded by the SEC14L2 gene (alternative names: C22orf6, KIAA1186, KIAA1658).
• It functions as a carrier protein that binds hydrophobic molecules with high affinity for alpha-tocopherol (vitamin E) and weaker affinity for other tocopherols and tocotrienols, facilitating their intracellular transfer.
• SEC14L2 also recognizes and binds squalene, potentially regulating cholesterol biosynthesis by shuttling squalene to a metabolically active pool within the cell.
• Association with alpha-tocopherol may confer transcriptional activatory activity on SEC14L2, linking lipid transport to gene expression regulation.
• Subcellular localization spans both the cytoplasm and the nucleus, aligning with its dual roles in metabolite delivery and transcriptional modulation.
• The protein is widely expressed, with notably high levels in liver, brain, and prostate tissues.
• SEC14L2 undergoes alternative splicing; its molecular weight is approximately 46 kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 60–270) targets a conserved region of SEC14L2, consistent with reported cross-reactivity across human, mouse, and rat orthologs.
• For Western blotting, a band near 46 kDa is expected; include appropriate positive and negative controls to confirm specificity.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize permeabilization and antigen retrieval protocols to account for the dual cytoplasmic/nuclear localization of SEC14L2.
• ELISA-based detection can employ this polyclonal antibody as a capture or detection reagent; validate performance with recombinant protein standards and blocking conditions.
• Being a polyclonal antibody, lot-to-lot consistency should be verified in pilot experiments prior to large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating lipid transport, vitamin E biology, or cholesterol regulation, we supply SEC14L2 antibodies alongside validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials. Leverage our sourcing expertise to secure rare target raw materials and streamline your assay development pipeline.