Anti-SEC14L2 Polyclonal Antibody for IHC-P, ELISA - O76054

Core Product Specifications and Parameters

Biological Background: SEC14L2 Function and Localization

• SEC14L2, also known as alpha-tocopherol-associated protein (TAP), supernatant protein factor (SPF), or squalene transfer protein, is a widely expressed carrier protein (UniProt: O76054).
• It binds with high affinity to alpha-tocopherol and with lower affinity to other tocopherols and tocotrienols, facilitating their transfer between cellular sites.
• SEC14L2 likely binds squalene, suggesting a role in regulating cholesterol biosynthesis by increasing the transfer of squalene into metabolically active pools.
• The protein may also possess transcriptional activatory activity through its association with alpha-tocopherol.
• Localization is primarily cytoplasmic and nuclear (SUBCELLULAR LOCATION: Cytoplasm, Nucleus).
• Widely expressed, with strong expression in liver, brain, and prostate (Tissue specificity).
• Multiple isoforms exist due to alternative splicing, reflected in observed molecular weights of approximately 36 kDa, 44 kDa, and 46 kDa.
• Keywords: Lipid-binding, transport, transcription regulation, activator, alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 1-275 amino acids of human SEC14L2, which may include tocopherol/squalene-binding domains; consider this when designing blocking peptides or epitope mapping.
• For IHC-P, standard antigen retrieval methods (e.g., citrate buffer pH 6.0, heat-induced) may be employed; optimise dilution and incubation conditions in the relevant tissue system.
• In ELISA applications, indirect or sandwich formats may be suitable; determine optimal coating concentration and secondary antibody conditions empirically.
• As a polyclonal antibody, it may recognize multiple isoforms; consider validating specificity in knockout or knockdown models if available.
• Given the predicted molecular weights (36, 44, 46 kDa), run adequate molecular weight markers and consider using nuclear and cytoplasmic fractions to confirm subcellular localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing for targets such as SEC14L2. Our supply and consulting solutions support every stage from concept to clinic, helping you accelerate development in lipid transport, cholesterol metabolism, and transcriptional regulation research. Reach out to explore how our integrated sourcing can streamline your workflow.