Anti-SEC13 Rabbit Polyclonal Antibody for WB, IHC, IF/ICC, ELISA - P55735

Core Product Specifications and Parameters

Biological Background: SEC13 Function and Localization

• Protein SEC13 homolog is a component of the nuclear pore complex (NPC) and the COPII coat, crucial for nucleocytoplasmic transport and ER-to-Golgi vesicle trafficking. Related references: PMID:8972206
• At the endoplasmic reticulum, SEC13 participates in the biogenesis of COPII-coated vesicles and is required for the efficient export of secretory proteins such as adipsin from the ER.
• As a core member of the GATOR2 complex, SEC13 positively regulates the mTORC1 signaling pathway by inhibiting the GATOR1 subcomplex. Related references: PMID:23723238, PMID:25457612, PMID:27487210, PMID:35831510
• In amino acid-rich conditions, the SEC13-containing GATOR2 complex likely catalyzes ubiquitination of NPRL2, leading to GATOR1 inactivation and mTORC1 activation. Related references: PMID:36528027
• SEC13 and its paralog SEH1L are required for the structural stability of the GATOR2 complex, ensuring optimal mTORC1 modulation. Related references: PMID:35831510
• SEC13 localizes to multiple cellular compartments including the cytoplasmic side of COPII vesicles, endoplasmic reticulum membrane, nuclear pore complex, and lysosome membrane.
• Post-translational modifications include acetylation and phosphorylation, and the protein undergoes alternative splicing.
• SEC13 contains multiple WD40 repeat domains, facilitating protein-protein interactions in multi-subunit complexes such as the NPC and GATOR2.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human SEC13 protein (residues 1–322). The antibody is expected to recognize all known isoforms generated by alternative splicing.
• For Western blotting, the predicted molecular weight is ~36 kDa. Use appropriate positive and negative controls to validate specificity in your sample system.
• In immunohistochemistry and immunofluorescence/ICC, permeabilization steps may be critical due to the membrane-associated and nuclear localizations of SEC13. Optimize fixation and permeabilization conditions.
• The antibody shows cross-reactivity with human, mouse, and rat samples; however, we recommend verifying reactivity in your specific model system.
• As a polyclonal reagent, lot-to-lot consistency should be monitored. Consider performing a bridging study when introducing a new batch into an established assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined one-stop sourcing experience for IVD raw materials, supporting your SEC13-related research and diagnostic development. In addition to this validated rabbit polyclonal antibody, we can supply matched antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Partner with CamelBio to bridge the gap from concept to clinic efficiently.