Anti-SEC11A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P67812

Core Product Specifications and Parameters

Biological Background: SEC11A Function and Localization

• SEC11A is the catalytic subunit of the signal peptidase complex (SPC), an ER-resident protease that cleaves N-terminal signal peptides from nascent secretory and membrane proteins.
• It specifically processes signal peptides with a hydrophobic h-region shorter than 18–20 amino acids. Related references: PMID:34388369
• The protein is anchored to the endoplasmic reticulum membrane via a signal-anchor sequence, with its active site facing the ER lumen.
• SEC11A is widely expressed and is essential for the maturation of proteins entering the secretory pathway.
• Known aliases include SPC18, SPCS4A, SEC11L1, and endopeptidase SP18, reflecting its 18–21 kDa molecular weight and membership in the SPC family.
• Post-translational modifications and alternative splicing may modulate its activity or interaction with other SPC subunits (UniProt keywords: Alternative splicing, 3D-structure).
• As a serine protease (hydrolase), SEC11A relies on conserved catalytic residues for signal peptide hydrolysis, a critical step in protein biogenesis.
• Key UniProt classifications: Endoplasmic reticulum, Hydrolase, Protease, Transmembrane helix, Reference proteome.

Experimental Guidance and Technical Tips

• For Western blot, the expected band size is approximately 21 kDa (precursor or mature form may show slight shifts). Use appropriate gel percentage and transfer conditions.
• In immunofluorescence/ICC, permeabilization is required to detect ER membrane localization; co-staining with ER markers is recommended.
• The immunogen corresponds to a region of human SEC11A (aa 37-145), which shares high homology with mouse and rat; cross-reactivity is expected but should be validated in the relevant sample system.
• For ELISA, consider using the antibody as a capture or detection reagent, depending on assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For SEC11A research, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research needs. Our team assists with rare target sourcing and assay development, ensuring seamless procurement and project progression.