Anti-SDSL Polyclonal Antibody for WB, ELISA - Q96GA7

Core Product Specifications and Parameters

Biological Background: SDSL Function and Localization

• SDSL (Serine dehydratase-like), also known as cSDH, SDH 2, or TDH, is a pyridoxal-phosphate-dependent enzyme that catalyzes the dehydrative deamination of L-serine and L-threonine to pyruvate and alpha-ketobutyrate, respectively. Related references: PMID:16580895 PMID:18342636
• It also exhibits glutamate racemase activity, interconverting L-glutamate and D-glutamate (By similarity).
• SDSL is predominantly expressed in lung cancer cell lines, linking it to tumor metabolism.
• The enzyme functions as a lyase and isomerase, and is involved in amino acid and lipid metabolism.
• It is subject to acetylation and contains a pyridoxal phosphate binding site.
• The molecular weight of the canonical isoform is approximately 35 kDa (theoretical: 34.7 kDa).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1–137 of human SDSL. This N-terminal region may include key catalytic residues; consider that the antibody may recognize epitopes within this domain.
• For Western blotting (WB), use appropriate cell or tissue lysates expressing SDSL, such as lung cancer cell lines, and validate the detected band at the expected molecular weight (~35 kDa).
• In ELISA applications, plate coating with the recombinant immunogen or peptide fragments can serve as positive controls.
• Given reported cross-reactivity with mouse, the antibody may be suitable for orthologous studies, but always verify in the relevant mouse sample system.
• Pyridoxal phosphate-dependent enzymes can be sensitive to reducing agents; optimize loading buffer conditions as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to supply a comprehensive range of IVD raw materials. This anti-SDSL polyclonal antibody supports metabolic and oncology research, complementing our portfolio of validated antibodies, bulk ancillary reagents, and rare target raw materials. From concept to clinic, we provide tailored sourcing solutions to accelerate your assay development.